why is empower only injecting one injection from sample set?

[seamoro]

im running a sample set and it is only injecting the first injection . it doesnt continue and inject the rest of the sample set...why is that?

[aceto_81]

any messages in your message center that may help?

[DR]

Did you specify a run time in the sample set method?

[seamoro]

well i have a gradient set up that stops at 15mins but the injection just keeps running....any ideas why?

[seamoro]

I still havent solved this one guys...please help

[aceto_81]

what runtime did you set in your sample set?
Do you use events in your instrument method?

[seamoro]

my run time is 15mins in the sample set.....i havent set any events...should i?

[seamoro]

ive talked with the technical support and they seem a bit clueless....

[aceto_81]

my run time is 15mins in the sample set.....i havent set any events...should i?

No you shouldn't, but just in case you had set some....
I think we need some more information:

Does your sample set stops after your first injection, or just keeps running without recording and going to the next injection?
Do you recieve any messages, in Empower's message center, or the windows event viewer?
What kind of instruments are we talking about: Alliance, GC, UPLC,....
Is this connected to a PC or a LACE?
Stand alone or Enterprise configuration?

Ace

[DR]

Add an event at 0.01min for the autosampler, turning switch 1 on. Move your start cable from the inject start over to switch 1. If the autosampler is not sending the start signal beyond the first injection of the sequence, this may work...

We had something similar going on w/ one of our 2695s and this worked. After a couple of days, we had a service engineer appt. setup and we put it back. It worked again once the engineer showed up - and has been working since. That's a good thing, because that set of contacts is attached to the main system board (expensive to replace).

[seamoro]

ok got its solved i believe.waters engineer installed a cable between back pda and sample manager.He said that normally you dont need this extra cable for aquity but in this case it did.cable is inserted into pda inject start jack. Thanks everybody for there help