ISTD Concentration

[dido79]

Good morning,
I'm new and I have no experience in gas chromatography. one of my questions is when preparing my ISTD (4-methyl-2-pentanol). I would need to prepare a solution having a concentration of 319.20 mg / l based on one liter bottle of 4-methyl-2-pentanol with MW = 102.18 g / mol and density 0.81g/cm3 whose purity is 98%.

Could you please help to resolve these calculations to prepare my ISTD.

Thank you very much and sorry for the inconvenience.

[Bigbear]

If the internal standard concentration is not specified in the method, I like to set the concentration in the middle of my calibration range.

[chromatographer1]

This sounds like a homework assignment.

98% of a weight of ISTD (x) should equal 319.2 mg to be put into a liter bottle. 0.98x = 319.2 x=319.2/0.98


ISTD density is 0.81g per cc

x (in grams) / 0.81g per cc = the volume of ISTD in cc (or mL)

best wishes,

Rod