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Buffers that Are Volatile And Have a Low UV Cutoff
Posted: Fri Aug 10, 2012 5:34 am
by adam
Hey all
I want to ask a question. Can anyone recommend buffers (or acids) that are both volatile and - at the same time - have a low UV cut off.
I can think of examples that fit one category or the other, but none that fit both.
Much Thanks
Re: Buffers that Are Volatile And Have a Low UV Cutoff
Posted: Fri Aug 10, 2012 6:00 am
by Mattias
There are no such buffers...
All volatile buffers are organic acids or salts thereof = signficant UV absorption below about 230 nm. The only option is too keep the buffer or acid concentration low, to be able to get a useful UV signal.
Re: Buffers that Are Volatile And Have a Low UV Cutoff
Posted: Fri Aug 10, 2012 12:20 pm
by zokitano
You can try with ammonium bicarbonate (NH4HCO3).
It has no UV absorption (> 210 nm) and is suitable for atmospheric pressure ionization sources for MS.
One important drawback: it is not suitable for preparation of low pH buffers
Re: Buffers that Are Volatile And Have a Low UV Cutoff
Posted: Tue Aug 14, 2012 3:54 pm
by adam
So there is really no option, if one needs to look at low wavelength UV and do evaporative light scattering detection at the same time (which requires a volatile buffer).
Re: Buffers that Are Volatile And Have a Low UV Cutoff
Posted: Wed Aug 15, 2012 11:08 am
by Gerhard Kratz
TFA, Acetic Acid, Formic acid, Acetate (K), Formate (K), Ammonium Bicarbonate 10mM could be used. Please remember that adding organic solvents UV-cut off is changed.