Hexane As A modifier in Polar organic solvent chromatography

[sandy1989]

Hi,
Im an Amateur chromatographer and i was trying to separate enantiomers of a drug on a lux chiral column by using polar organic solvent chromatography and i tried many permutations and combinations of the organic solvents, and at some point i was able to achieve a nice peak resolution with a symmetric peak shape only after using hexane as a modifier.
Now my question is whether can we use hexane as a modifier in POSC? and let me tell u that there are no miscibility issues in there and i used only 10% of Hexane and the mobile phase contains MeCN and MeOH

[danko]

Use hexane and be happy.
Best Regards

[sandy1989]

First of all.. thanks fr the reply
Danko
and am using Hexane and its good, but i need a help..... is there any method which uses Hexane and MeCN at a time in a mobile phase for HPLC

[danko]

I'm sure there is/are.

[sandy1989]

I tried a lot for the literature but couldn't find a single paper which uses both Hexane and MeCN as a mobile phase.
But if u have one can u pls provide me the link Danko... thanks in advance

[Csaba]

Use iso-hexane and not n-hexane due to SHE issue.
And iso-hexane is much cheaper than n-heptane.
It works very well.

[danko]

Hi sandy1989,

I can't provide a link to such a method and unfortunately I don't have the time to do a search.
But I can not - in my wildest fantasies - imagine that there aren't any instances.

best Regards

[sandy1989]

Well Thanks for ur valuable time and suggestion danko....

[HPLCaddict]

Just for a start:
http://bit.ly/15BBg4x

I don't have access to the fulltext, but "The effects of trifluoroacetic acid (TFA) and n-hexane concentration in an acetonitrile (MeCN) mobile phase were investigated" sounds like something you might be looking for.

[sandy1989]

Thanks HPLCaddict and I have this article already but even I forgot that ,... it was helpful not totally but as u said its a Start... .

[adam]

Csaba:

Can you please explain what you meant by this: "Use iso-hexane and not n-hexane due to SHE issue."

Thanks!!

[sandy1989]

SHE is Safety Health Environment.....

[Csaba]

SHE = Safety Health Environment - common aberration in the industry. n-Hexane is cancerogenic and you have to have a good reason to use it otherwise it is NO.

[uzman]

Hi Csaba ,

I couldn't locate any Material Safety Data that mentions n-hexane is carcinogen.

OSHA , NTP and IARC classifies at not carcinogen.
NFPA Rating for Health=2

[sandy1989]

Hi follow the link below

http://www.atsdr.cdc.gov/toxfaqs/tf.asp?id=392&tid=68

its not carcinogenic but inhalation causes nerve damage and paralysis of the arms and legs