Buffers that Are Volatile And Have a Low UV Cutoff

[adam]

Hey all

I want to ask a question. Can anyone recommend buffers (or acids) that are both volatile and - at the same time - have a low UV cut off.

I can think of examples that fit one category or the other, but none that fit both.

Much Thanks

[Mattias]

There are no such buffers...

All volatile buffers are organic acids or salts thereof = signficant UV absorption below about 230 nm. The only option is too keep the buffer or acid concentration low, to be able to get a useful UV signal.

[zokitano]

You can try with ammonium bicarbonate (NH4HCO3).
It has no UV absorption (> 210 nm) and is suitable for atmospheric pressure ionization sources for MS.
One important drawback: it is not suitable for preparation of low pH buffers

[adam]

So there is really no option, if one needs to look at low wavelength UV and do evaporative light scattering detection at the same time (which requires a volatile buffer).

[Gerhard Kratz]

TFA, Acetic Acid, Formic acid, Acetate (K), Formate (K), Ammonium Bicarbonate 10mM could be used. Please remember that adding organic solvents UV-cut off is changed.