Chromatography Forum

may you show the progress of lactic acid analysis method using post-cloumn derivatization

You can analyze lactic acid directly at low UV with phosphoric, sulfuric or other UV transparent acid and Primesep B2 column. Lactic acid has several oligomers and you can separate all of them with this method:

http://hplcmethods.com/compound_156.php

Regards,

Vlad

I doubt you will find much using post-column reaction. If you do, it is almost certainly an enzymatic method.

Detection by UV at 210 nm or conductivity are usually adequate. The two reasons one might choose detection using post-column reaction are 1) a need for better sensitivity and/or 2) a need for more selective detection. Do any of these apply to your situation?

detect the lactic acid, acetic acid, propionic acid, butyric acid using SHIMADZU LC-10A and SPD-10A (UV) Detector, the column is Dikma Rspak KC-811.
If the post-column reaction is necessary. If not, the optimal process is ?

What are the concentrations of these acids you need to measure? What kind of sample matrix are they in?

the concentrations of every acid is about 0.01%~0.1%.
the water solution

Using UV at 210 nm, the detection limit is around 10 mg/L; you should have no problem in your concentration range. If there are no interfering substances in your water, and your column can resolve everything, you need only your UV detector.

Try my best with your purpose.
Regards
Xu qingfang