Fluorescence reapeatability
Posted: Mon Jul 02, 2012 12:01 pm
Hello
I´m working on agilent 1100 system with DAD and fluorescence detectors. My coworker had some suspicions about the FL results and i made 4 parallels with vitamin E(10mg/l). Results form dad:
8,32431
8,28639
8,11963
8,76863
relative std: 3,36%
FL:
8,74104
9,34229
10,35499
8,45234
relative std: 8,87%
I don´t have alot of experience with those detectors but the std of fluorescence detector seems too high. Is this a problem and if it is how can I determine the problem?
I´m working on agilent 1100 system with DAD and fluorescence detectors. My coworker had some suspicions about the FL results and i made 4 parallels with vitamin E(10mg/l). Results form dad:
8,32431
8,28639
8,11963
8,76863
relative std: 3,36%
FL:
8,74104
9,34229
10,35499
8,45234
relative std: 8,87%
I don´t have alot of experience with those detectors but the std of fluorescence detector seems too high. Is this a problem and if it is how can I determine the problem?