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- Posts: 1
- Joined: Thu Aug 23, 2012 5:21 am
I would like some input on capillary voltage with electrospray.
In "Electrospray: from ions in solution to ions in the gas phase, what we know now" (Kebarle and Verkerk, Mass Spectrometry Reviews, 2009, 28, 898-917) you can read about required voltage for electrospray (p 901) - theoretical and experimentally verified. The lowest capillary voltage stated is 2,2 kV, and that is for acetonitrile with low surface tension.
The "problem" is that my expreience tells me otherwise. Ten years ago we optimized out methods with syringe infusion only, i.e. 10 µL/min. Back then a capillary voltage of about 3 kV was optimal. When we started to use higher flow rates through a tee and mixed with syringe infusion the optimal voltage decreased to 1 kV or less. And nowdays three out of ten methods have capillary voltage above 2 kV, the rest is optimal in the range 0,3-1 kV. We have Quattro Micro and Xevo.
Anyone with the same experience? Other ideas, views, concerns?
In "Electrospray: from ions in solution to ions in the gas phase, what we know now" (Kebarle and Verkerk, Mass Spectrometry Reviews, 2009, 28, 898-917) you can read about required voltage for electrospray (p 901) - theoretical and experimentally verified. The lowest capillary voltage stated is 2,2 kV, and that is for acetonitrile with low surface tension.
The "problem" is that my expreience tells me otherwise. Ten years ago we optimized out methods with syringe infusion only, i.e. 10 µL/min. Back then a capillary voltage of about 3 kV was optimal. When we started to use higher flow rates through a tee and mixed with syringe infusion the optimal voltage decreased to 1 kV or less. And nowdays three out of ten methods have capillary voltage above 2 kV, the rest is optimal in the range 0,3-1 kV. We have Quattro Micro and Xevo.
Anyone with the same experience? Other ideas, views, concerns?
