Calibration curve when no blank sample
Posted: Thu Jun 28, 2012 4:02 am
Hi, allI have questions regarding calibration curves. When the calibration curve prepared by standard solution always give lower amount of analyte than the actual, matrix match calibration is use to compensate the matrix influence. A blank sample is needed for matrix match calibration; unfortunately, biological samples always contain compound of interests. So, what sample should I consider as blank sample (in my case- I need rice without tocopherols)? I am figuring out
what will be the sample after read a journal saying this ''The tocols in the extracts were quantified by a matrix-standard calibration with extracts from pooled cereal sample spiked with increasing amounts of the standards solutions at different concentrations.'' Let say, I could not find rice without tocopherols, can I use the calibration curve produced by standard addition method using one representative rice sample to quantify tocopherols amount in different rice samples? Is this possible and valid? Your help/idea is highly needed and deeply appreciated. Thank You
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