Purchase of HPLC

[Rana]

Hi everybody
I will buy an HPLC coupled to a diode array detector.
I hesitate between a Dionex Ultimate 3000 (LPG-3400SD pump, autosampler WPS-3000SL, oven TCC-3000SD, diode array detector DAD3000, Chromeleon software) and a Waters Alliance (e2695 Separations Mod. W / CH, detector 2998 diode array, Empower 3).
What do you recommend?
What do you think of the detector Dionex DAD-3000?
And what do you think of the pump waters?

thank you very much

[rwang]

Hi everybody
I will buy an HPLC coupled to a diode array detector.
I hesitate between a Dionex Ultimate 3000 (LPG-3400SD pump, autosampler WPS-3000SL, oven TCC-3000SD, diode array detector DAD3000, Chromeleon software) and a Waters Alliance (e2695 Separations Mod. W / CH, detector 2998 diode array, Empower 3).
What do you recommend?
What do you think of the detector Dionex DAD-3000?
And what do you think of the pump waters?

thank you very much

I have not used Dionex U3000 but I've been a user for e2695/2998 PDA for a while and it's very reliable. There has not been any major issue and the pump is trouble free (we had it under service contract but so far no major replacements have been done).

The pumps are rated 350bar but can be pushed further via third party modifications. Note that I have not done this and by doing that will certainly void the manufacturer's warranty. The autosampler of alliance 2695 uses a side-draw needle which requires special vials to get total recovery of samples. The injection cycle is also a bit long by today's standards.

I would recommend you considering the Waters Acquity H-Class since it enables you to use "sub-2" columns and the dwell volume is substantially lower. Though it has an FTN-type sampler, it uses a bottom draw needle.

As far as I know the alliance autosampler cannot handle online sample derivatisation, which both Waters Acquity H-Class and Dionex U3000 are capable of, but I will stand corrected if I'm wrong.

I'd like to say every HPLC/UHPLC has its limitations, be it pressure limit, flow rate or price tag Let your fellow chromatographers on this forum know your intended purpose for the HPLC so they can better advice you.

Disclaimer: I do not work for Waters or Dionex and I am no salesperson.

Cheers,

[gstaepels]

Rana,

I have been using Waters equipment for about 25 years and I like itvery much. The Empower software may seem complicated at the beginning, but as you get used to it, it is very good. The equipment itself may be more expensive that others at the time of purchase, but at the end of the day, it will show the cheapest, since the low amount of service calls, and the life span. I used a system for over 20 years, and have descided to replace it, just because we wanted to take advantage of the latest technology. BTW, the latest system is an Alliance e2695 with UV and RI detectors.

[Bluesman75]

Hi everybody
I will buy an HPLC coupled to a diode array detector.
I hesitate between a Dionex Ultimate 3000 (LPG-3400SD pump, autosampler WPS-3000SL, oven TCC-3000SD, diode array detector DAD3000, Chromeleon software) and a Waters Alliance (e2695 Separations Mod. W / CH, detector 2998 diode array, Empower 3).
What do you recommend?
What do you think of the detector Dionex DAD-3000?
And what do you think of the pump waters?

thank you very much

Hi Rana, we have recently got a Dionex 3000 ultimate UPLC and so far we are very happy with it and the software (massive step up from our ChemStation/ChemStore setup). The UPLC is very easy to use (but all HPLC/UPLC should be these days), but I would say make sure you read the manuals and get some familiarisation training off the engineer that installs it. There were some bits that were new to me as I come from and Agilent 1100 back ground.

The software is very easy to use. All the familiar Windows type functions (right clicks) all do what you expect them to. Myself and a less IT confident/literate colleague have picked it up very easily.

I would say that Dionex haven't been that great following the install. I have had to do more chasing than I would like to get queries & additional quotes followed up.

Your best bet is to write out a list of your requirements and get some demos and quotes organised.

Cheers
Mike

[Hfranz]

As rwang already stated, the Alliance is a reliable but also a different generation instrument. It will be very difficult if not impossible to benefit from modern UHPLC separations. Modern HPLC or UHPLC instruments for instance provide reduced gradient delay volumes and extra column volumes. Higher pump pressure levels allow you to run both conventional and UHPLC applications. There are differences between modern LC systems, depending on your personal requirements and application needs. In the end you should get your hands on the instrument and the software to make a well-grounded choice, so to ask for a demo is definately a good idea. If you should have technical questions about the UltiMate 3000 system, feel free to contact me directly.

Holger
-----------
Dionex (part of Thermo Fisher Scientific)

[remesquaddie]

We look after many Waters Alliance systems, 2695 with 2996, 2998. They are very reliable . We see next to nothing in Varian products in the major labs, so I cannot comment on their performance.

[rwang]

Oh, and don't forget the software package and technical support.

Though I'm an Empower user, I have heard of good things from both Empower and Chromeleon CDS. As software has huge impact on your productivity, you may want to pick the one that you are most comfortable with, choices between different CDS packages can be very personal.

Even the best HPLC/UHPLC needs to be serviced and maintained at certain intervals and it is very important to know the level of the vendor's technical support IN YOUR AREA. Choose the one with good local support.

[fareedtayyeb]

Hi there !!!
Did you purchased Waters LC unit ??
Are you from Pakistan ??

Best regards and have a nice time

Fareed Tayyeb

Hi everybody
I will buy an HPLC coupled to a diode array detector.
I hesitate between a Dionex Ultimate 3000 (LPG-3400SD pump, autosampler WPS-3000SL, oven TCC-3000SD, diode array detector DAD3000, Chromeleon software) and a Waters Alliance (e2695 Separations Mod. W / CH, detector 2998 diode array, Empower 3).
What do you recommend?
What do you think of the detector Dionex DAD-3000?
And what do you think of the pump waters?

thank you very much

[fareedtayyeb]

Dear Sir,
Actually the problem is that only 3 Brands have a greater strength in this part of world. It is between Agilent,Shimadzu and Waters. Otherthan are good but do not have capacity to support successfully.
Waters is good enough comparing to Shimadzu. Agelint is also good in specs but they have also have repute of not performing services at highest level.
Regds
Tayyeb

Hi there !!!
Did you purchased Waters LC unit ??
Are you from Pakistan ??

Best regards and have a nice time

Fareed Tayyeb

Hi everybody
I will buy an HPLC coupled to a diode array detector.
I hesitate between a Dionex Ultimate 3000 (LPG-3400SD pump, autosampler WPS-3000SL, oven TCC-3000SD, diode array detector DAD3000, Chromeleon software) and a Waters Alliance (e2695 Separations Mod. W / CH, detector 2998 diode array, Empower 3).
What do you recommend?
What do you think of the detector Dionex DAD-3000?
And what do you think of the pump waters?

thank you very much