Chromatography Forum

Hi,

I'm almost new in the HPLC field, and I need help so much I would like
I'm going to develop a method for the quantification of Terlipressin in solution by HPLC-UV. I've found an article that uses RP18 column with the follow mobile phase: Acetonitril: monobasic potassium phosphate solution (35:45) (monobasic potassium phasphate solution prepared by dissolving 1400mg KH2PO4 in 100mL double distilled water).

But I'm not really sure that the mobile phase composition is adequate. May be too much concentrated????????

Could somebody help me with it?
Do you know some method for terlipressin quantification?

Thanks!!

1400 mg/100 mL = 1.4 g/100 mL = 14g/L
The molecular weight of KH2PO4 is 136, so 14g/L = 0.1M = 100mM

A bit on the high side (most HPLC methods use buffers in the range from 10-50 mM) but it's not outrageous.

Thanks at all Tom!!!