Agilent 1100, icons green, stable baselines, press start...

[incitatus1973]

and nada....the software flips back to "ready" status without actually starting the run....I'm not a great technician and I use the HPLC as means to an end. Any help would be super appreciated.

P.s. just starting the troubleshooting which is why I have not included too much information....everything was working great until I constructed a new method and changed from RID to DAD....but the DAD icon is green and the baseline looks completely fine....is there something I missed?

[antonk]

Maybe just analysis length (gradient program length) became 0 and instrument decides better not to run.

[agoupax]

Have you tried to close the software and reboot everything (computer and all LC modules alike)?

[Bluesman75]

What software are you using? Most have some sort of log that may provide some clues.
Also does the method you ran previously, before swapping detectors still run?

[warlock4761]

So you are running on DAD, using EZ Chrome software? Does the status at the bottom say "Waiting for Equilibration"?