Unichrom walk-through/demo/step-by-step please!
Posted: Tue Jun 19, 2012 2:17 am
by ChristoListo
Hey folks, I am rather confused with Unichrom. Does someone have the patience to give me a detailed step-by-step instruction on how to do something like calibration? All I want to do, is use an internal standard to estimate the abundance of a similar chemical on the same trace. I know this question is a long shot, but it would help get the ball rolling for me.
Re: Unichrom walk-through/demo/step-by-step please!
Posted: Wed Jun 20, 2012 4:13 pm
by antonk
Here is users manual
http://www.unichrom.com/chrom/ug5eng.pdf
There is chapter "Shortest way to Aim".
To get your result with internal standard:
1. Integrate (get the peak with calculated areas): F9 - "Peak Search"
2. name your internal standard (double click peak title) or fill the "Peaks" table.
3. Calculate: F9 - Calculation/Internal standard (select the name of ISTD and its concentration).
Also have a look to prepared solutions (most of them have textual description in .doc file):
http://www.unichrom.com/solutionse.shtml
http://www.unichrom.com/solutions/Inter ... %20eng.zip
Do not hesitate ask your questions to unichrom at unichrom dot com