Chromatography Forum

and nada....the software flips back to "ready" status without actually starting the run....I'm not a great technician and I use the HPLC as means to an end. Any help would be super appreciated.

P.s. just starting the troubleshooting which is why I have not included too much information....everything was working great until I constructed a new method and changed from RID to DAD....but the DAD icon is green and the baseline looks completely fine....is there something I missed?

Maybe just analysis length (gradient program length) became 0 and instrument decides better not to run.

Have you tried to close the software and reboot everything (computer and all LC modules alike)?

What software are you using? Most have some sort of log that may provide some clues.
Also does the method you ran previously, before swapping detectors still run?

So you are running on DAD, using EZ Chrome software? Does the status at the bottom say "Waiting for Equilibration"?