mobile phase issue

[dogiparthi.sudhir]

why mobile phase getting hazy below 20 degrees temp

mobile phase is SLS

[KM-USA]

lessened solubility?

[dogiparthi.sudhir]

but at room temp its stable

[HPLCaddict]

Sounds as if you're just at the edge of solubility at room temperature. A few degrees down at the SLS starts precipitating:(

[juanantonio]

but at room temp its stable

Its not about stability only, it may be due to solubility. The solubility of a compound also differs at different environment and temperature may be a factor. Try to warm up the mobile phase once you get hazy and see the difference. If the soltion becomes clear, may be its the temperature.

[prepcolumn]

Are you using any potassium salt in your mobile phase?

[Klaus I.]

why mobile phase getting hazy below 20 degrees temp

mobile phase is SLS

Sorry I'm not a natural speaker: What means 'SLS'?

[dogiparthi.sudhir]

why mobile phase getting hazy below 20 degrees temp

mobile phase is SLS

Sorry I'm not a natural speaker: What means 'SLS'?

SLS Sodium lauryl sulfate

[Vlad Orlovsky]

I suggest that you switch to mixed-mode where IP reagent is attached to the surface.....or add a little bit of THF to your mobile phase

[dogiparthi.sudhir]

Are you using any potassium salt in your mobile phase?

we are using sodium dihydrogen phosphate anhydrous