Chromatography Forum

Posted: **Tue Jun 05, 2012 10:32 pm**

why mobile phase getting hazy below 20 degrees temp

mobile phase is SLS

Posted: **Tue Jun 05, 2012 10:58 pm**

lessened solubility?

Posted: **Tue Jun 05, 2012 11:01 pm**

but at room temp its stable

Posted: **Wed Jun 06, 2012 7:56 am**

Sounds as if you're just at the edge of solubility at room temperature. A few degrees down at the SLS starts precipitating:(

Posted: **Wed Jun 06, 2012 10:34 am**

but at room temp its stable

Its not about stability only, it may be due to solubility. The solubility of a compound also differs at different environment and temperature may be a factor. Try to warm up the mobile phase once you get hazy and see the difference. If the soltion becomes clear, may be its the temperature.

Posted: **Wed Jun 06, 2012 2:37 pm**

Are you using any potassium salt in your mobile phase?

Posted: **Wed Jun 06, 2012 3:58 pm**

why mobile phase getting hazy below 20 degrees temp

mobile phase is SLS

Sorry I'm not a natural speaker: What means 'SLS'?

Posted: **Wed Jun 06, 2012 7:25 pm**

why mobile phase getting hazy below 20 degrees temp

mobile phase is SLS
Sorry I'm not a natural speaker: What means 'SLS'?

SLS Sodium lauryl sulfate

Posted: **Wed Jun 06, 2012 8:10 pm**

I suggest that you switch to mixed-mode where IP reagent is attached to the surface.....or add a little bit of THF to your mobile phase

Posted: **Thu Jun 07, 2012 9:52 am**

Are you using any potassium salt in your mobile phase?

we are using sodium dihydrogen phosphate anhydrous

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