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HPLC method for sara analysis
Posted: Tue Jun 05, 2012 4:37 pm
by Muhammad Akbar Khan
Hi everyone,
I am using Infinity 1260 series by agilent. I do not have HPLC method for sara (saturates, aromatics, resins and asphaltene) analysis of crude oil. I need a HPLC method for sara anylsis, can you people help me out?
thanks and regards
Akbar
Re: HPLC method for sara analysis
Posted: Thu Jun 28, 2012 2:52 am
by vnaren
I suppose your sample is crude oil. First dissolve the sample in hexane to precipitate out asphaltenes from the rest and recovered it by filtration.
Inject the saturates and aromatics onto NH2 column with hexane as mobile phase. Saturates and aromatics peaks can be quantified.
Resin is retained strongly and did not elute with hexane. 30%DCM/hexane backflush needs to be used to elute resin.
You can refer to the article
"Rapid and Accurate SARA Analysis of Medium Gravity Crude Oils" for details.
Hope this helps.
Re: HPLC method for sara analysis
Posted: Thu Jun 28, 2012 3:20 pm
by Muhammad Akbar Khan
Thanks vnaren,
Yes I want to do same, but I did not run the analysis on this intrument as I do not have parameters to set it. I also need to know how to set back flush and which column we need to install for this?
Can you guide me in this
Kind regards