Primary amine derivatization for fluorescence detection
Posted: Mon Jun 04, 2012 11:45 am
Hi,
I have a HPLC method for quantification of certain compound using OPA/MPA pre-column derivatization and fluorescence detection. Because OPA/MPA is not stable enough I need to use elaborate injector program to derivatize sample just before injecting it into HPLC and problem with it is that I cannot transfer it to other (new) system. What I am thinking is that perhaps I could use different derivatization chemistry?
Requirements would be:
1. Must be stable for at least 24 hours.
2. Must be reactive only towards primary amines (my compound has another group with secondary-like properties - learned that when AccQtag chemistry gave two products).
3. Must be at least same sensitivity as OPA/MPA (it's a trace analysis).
Any suggestions? Or idea of source where I could find a bunch of different chemistries with information on stability and sensitivity?
(Note: edited for clarity.)
I have a HPLC method for quantification of certain compound using OPA/MPA pre-column derivatization and fluorescence detection. Because OPA/MPA is not stable enough I need to use elaborate injector program to derivatize sample just before injecting it into HPLC and problem with it is that I cannot transfer it to other (new) system. What I am thinking is that perhaps I could use different derivatization chemistry?
Requirements would be:
1. Must be stable for at least 24 hours.
2. Must be reactive only towards primary amines (my compound has another group with secondary-like properties - learned that when AccQtag chemistry gave two products).
3. Must be at least same sensitivity as OPA/MPA (it's a trace analysis).
Any suggestions? Or idea of source where I could find a bunch of different chemistries with information on stability and sensitivity?
(Note: edited for clarity.)