Chromatography Forum

Hello!
I'm trying to set up my first GC method ever, targeting glycerol. I'm using the method described in the current Ph.Eur, but I'm experiencing poor precision. No doubt due to the injection split mode!

My question is if anyone could suggest a proper and readily available internal standard suitable for analysis of glycerol in aqueaus samples!

Thanks,

CSV

Hi CSV,

The choice you can do is the diethylene glycol as EI (or maybe one butyl glycol)
Principal is that your EI have the same physico- chimical behaviour as your glycerol.

We've published on glycerin quantitation by GC, do it all the time. We usually do by external standard after dissolving sample in DMF (up to 2.5% sample) then derivatizing with BSTFA. When we have done internal standard quantitation we've used butylene glycol, but propylene glycol and ethylene glycol would also deliver one peak each and be OK. Like previous poster, these have similar characteristics to the sought-for analyte.