Chromatography Forum

Hello,

I'm currently developing a method for the determintation of Alternaria toxins and am having difficulties
getting proper retention for tenuazonic acid (TeA). I've heard of methods using PFP-Phases but would like to know
if anybody out there has any experience with TeA and if so what your suggestions are.

Thank in Advance
Alex

J Assoc Off Anal Chem. 1980 May;63(3):612-21.
Liquid chromatographic determination of tenuazonic acids in tomato paste.
Scott PM, Kanhere SR.
Abstract
Tenuazonic acid is a mycotoxin produced by Alternaria alternata (A. tenuis) occurring in isolates from tomato. Several liquid chromatographic (LC) systems were compared for suitability in analyzing tomato pastes for tenuazonic acid. Tenuazonic acid was extracted from tomato paste with methanol and n-hexane and was partitioned into methylene chloride from the aqueous methanol following acidification. It was further partitioned into 5% NaHCO3, acidified, and reextracted with methylene chloride. LC separation of tenuazonic acid from interferences was best on a C12-dien (4-dodecyldiethylenetriamine) loaded C18 reverse phase column with a mobile phase containing C12-dien, Zn(II), and ammonium acetate, or on a tetraalkylammonium anion exchange column at pH 7.5. Two isomeric tenuazonic acids could be partially separated in the C12-dien/metal system using acetonitrile in the mobile phase. Amounts of standard tenuazonic acid as low as 2-5 nm. Recoveries of tenuazonic acid or isotenuazonic acid added to tomato paste at levels of 0.4-10 micrograms/g were 67-94%. Small amounts of tenuazonic acid were detected in several commercial tomato pastes at levels of 0.01-0.1 micrograms/g. Gas liquid chromatography/mass spectrometric (GLC/MS) single ion monitoring showed that both isomers were present.

Hi and thanks for the quot!

I'll see if I can find a comparable column somewhere!