Hi there,

I have lysed my E. coli culture using PopCulture reagent from Millipore.

Now I have to quantify a protein, and I want to use LC/MS/MS. I am aware that some detergents (eg SDS) will interfere with LC/MS/MS measures (when not killing the column); I was wondering whether anyone has tried before to run LC/MS/MS after PopCulture Reagent and whether there are protocols in place to clean the sample before tripsination.

Many thanks,

Miche