PopCulture Reagent cell lysis and LC/MS/MS protein quant?
Posted: Mon May 21, 2012 4:01 am
Hi there,
I have lysed my E. coli culture using PopCulture reagent from Millipore.
Now I have to quantify a protein, and I want to use LC/MS/MS. I am aware that some detergents (eg SDS) will interfere with LC/MS/MS measures (when not killing the column); I was wondering whether anyone has tried before to run LC/MS/MS after PopCulture Reagent and whether there are protocols in place to clean the sample before tripsination.
Many thanks,
Miche
I have lysed my E. coli culture using PopCulture reagent from Millipore.
Now I have to quantify a protein, and I want to use LC/MS/MS. I am aware that some detergents (eg SDS) will interfere with LC/MS/MS measures (when not killing the column); I was wondering whether anyone has tried before to run LC/MS/MS after PopCulture Reagent and whether there are protocols in place to clean the sample before tripsination.
Many thanks,
Miche