Column overpressure
Posted: Thu May 17, 2012 10:06 am
Hi
I've been working on an experiment in which my mobile phase is a gradient method consisting of acetonitrile and buffer (ammonium acetate in water, pH 4.6). I am using an Inertsil ph-3 10 cm column, and it runs fine, but when I want to run it again after a completed sampleset, the system goes over pressure. This happened with two columns now. At the end of the first succesfully completed sampleset i washed the column with Acetonitrile:water (1:1) for 60 minutes, and even now I have been washing it with methanol, water mixtures, acetonitrile mixtures and even reversed the column, yet nothing seems to help. Any help would be great!
I've been working on an experiment in which my mobile phase is a gradient method consisting of acetonitrile and buffer (ammonium acetate in water, pH 4.6). I am using an Inertsil ph-3 10 cm column, and it runs fine, but when I want to run it again after a completed sampleset, the system goes over pressure. This happened with two columns now. At the end of the first succesfully completed sampleset i washed the column with Acetonitrile:water (1:1) for 60 minutes, and even now I have been washing it with methanol, water mixtures, acetonitrile mixtures and even reversed the column, yet nothing seems to help. Any help would be great!