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Higher Basline

Posted: Mon May 14, 2012 8:15 am
by Davidantony
Dear All,
I am developing a multi Pesidue method in API-2000.for one of the compound base line started at 1.0 e4.how this higher baseline comes any one know how to remove this problem please reply

Re: Higher Basline

Posted: Mon May 14, 2012 10:09 am
by Jetjamnong
Flush the HPLC column with a minimum HPLC grade Acetronitrile gradiently overnigth, then check your baseline again.