Change FPLC to HPLC for ssDNA seperation

[harish]

Hallo,
i have a FPLC AKTA from amersham. I want to use it for speration of single strand DNA 20-100 bases. I have a problem of pressure as it cant go more than 10 Mpa. Is it enough to use a better pump or longer column. I dont see any peaks in the graph. Or there is no option than replacing with new HPLC which is costly.
Please help me in this regard,
Greetings,
Harish.

[megranate16]

you should find the suitable column for the ssDNA seperation. and according to the column and packing material requirement choose the equipment.



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[unmgvar]

what is the the column and conditions you want to use?
do you want to have a good separation between n to n-1?
are these "end protected" ssDNA?