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syringe filter
Posted: Thu May 03, 2012 6:32 pm
by jiangds06
how do I choose syringe filter in order to minimize adsorption of hydrophobic analytes in aqueous phase? nylon? PTFE? GSH?
Re: syringe filter
Posted: Thu May 03, 2012 7:21 pm
by JGK
For Aqueous Phases PTFE or PP filters are not normally recommended.
you may have to run some comparative work with other types listed in the selection guides
http://www.chromtech.com/Catalog7/PDF_Indvpge/284.pdf
Re: syringe filter
Posted: Fri May 04, 2012 5:31 am
by Alexandre
There are recommendations, but for the final tuning you may need to do experiments.
Also watch for leaching interferences. Ultimately – centrifuge.
Re: syringe filter
Posted: Tue May 08, 2012 6:40 pm
by jiangds06
this selection guide is for protein. do you have selection guide based on small molecules.
Anyway, hydrophobic interaction is responsible for protein binding, right?
Re: syringe filter
Posted: Fri May 11, 2012 3:29 pm
by KM-USA
For aqueous samples we typically use nylon or glass membrane filters, such as GE Whatman Autovials. But we do check for recovery/interferences.
For organic or mixed organic-aqueous, we typically use PTFE.
Re: syringe filter
Posted: Fri May 11, 2012 4:27 pm
by krickos
In worst case you may also have to consider the syringe (plastic-glass), not often but happned to us that plastic disposeble syringe adsorbs the analyte.