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Ion chromatography for dummies
Posted: Fri May 20, 2005 5:59 am
by orcicdejan
We want to start analyzing anions by ion chromatography (we have Agilent's equipment). Any tips & tricks? How to prepare optimal mobile phase? Does anybody know what is composition of Agilent's 'mobile phase modifier'?
Thanks in advance!
Ion Chromatography
Posted: Fri May 27, 2005 7:00 pm
by Alain
You find it difficult to analyze Anions with a stainless steel system. PEEK would be best since eluents(mobile phases) are NaOH or Carbonate/Bicarbonate
Posted: Fri May 27, 2005 7:53 pm
by tom jupille
Actually, Alain, anions can be analyzed quite well in SS systems (trust me on this, I used to do a lot of it!
).
orcicdejan, are you following a specific procedure from Agilent, or are you starting from scratch?
Posted: Fri May 27, 2005 8:01 pm
by Alain
Good luck with that
Posted: Mon May 30, 2005 1:58 am
by Yury Zelechonok
If you can add ELS detector to your Agilent then many cations and anions can be analysed with simple technology discribed in
http://allsep.com/brochures/PrimesepIC_Rev3.pdf
Posted: Tue Jun 07, 2005 9:24 am
by orcicdejan
I'm using conditions recommended in Agilent's HP Anion Chromatography System User's Guide: mobile phase 810mL H2O + 50mL modifier + 140mL ACN + NaOH until pH=8,6, flow 1,5mL/min, oven 40"C, det UV 360/20nm vs 266/10nm. Column is Agilent-IC (Asahipak ODP-50 125x4mm). I've obtained good separation and sensitivity for F-, Cl-, SO4 2-, HPO4 2-, but signal for I- is too weak (for concentrations comparable to other ions, signal is ~10x weaker).
Ion chromatography for dummies
Posted: Mon Jun 13, 2005 11:58 pm
by Chris Pohl
orcicdejan,
It looks like you are using indirect UV detection. In this mode you will not get good detection sensitivity for UV absorbing analytes such as iodide. Iodide is easy to detect via direct UV detection with a UV transparent eluent.