syringe filter

[jiangds06]

how do I choose syringe filter in order to minimize adsorption of hydrophobic analytes in aqueous phase? nylon? PTFE? GSH?

[JGK]

For Aqueous Phases PTFE or PP filters are not normally recommended.

you may have to run some comparative work with other types listed in the selection guides http://www.chromtech.com/Catalog7/PDF_Indvpge/284.pdf

[Alexandre]

There are recommendations, but for the final tuning you may need to do experiments.
Also watch for leaching interferences. Ultimately – centrifuge.

[jiangds06]

this selection guide is for protein. do you have selection guide based on small molecules.
Anyway, hydrophobic interaction is responsible for protein binding, right?

For Aqueous Phases PTFE or PP filters are not normally recommended.

you may have to run some comparative work with other types listed in the selection guides http://www.chromtech.com/Catalog7/PDF_Indvpge/284.pdf

[KM-USA]

For aqueous samples we typically use nylon or glass membrane filters, such as GE Whatman Autovials. But we do check for recovery/interferences.

For organic or mixed organic-aqueous, we typically use PTFE.

[krickos]

In worst case you may also have to consider the syringe (plastic-glass), not often but happned to us that plastic disposeble syringe adsorbs the analyte.