HILIC separations - can they be modelled by DryLab?

[Rob Burgess]

.... or is the separation mechanism too complex to understand or does not follow a strict linear solvation energy relationship?

Also, is it possible to perform gradient separations with HILIC?

[tom jupille]

To take those in reverse order:

Yes, gradients can be used in HILIC. Phenomenex has a good example on page 14 of their Luna HILIC brochure:
http://tinyurl.com/7mcwy5n

Re DryLab, HILIC is essentially normal-phase liquid-liquid partition chromatography. The "normal phase" mode of DryLab should do OK for that. If you want to play with it, the DryLab 2000 versions had a "custom mode" capability that would let you pick your own fitting function (I'm ot sure if that carried over into the later versions, though).