Hi everybody!

We need to develope a HPLC method for the determination of vitamin D3 in the dissolution test for alendronic acid/cholecalciferol tablets (70mg/2800 UI). The FDA mentions the conditions for the dissolution test for both actives. In the case of cholecalciferol the medium is 0,3% SDS in water and the volumen is 500 mL. Does anybody know about an uv HPLC method for this type of assay?

Thank you very much in advance!