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IS for Tricyclic Antidepressants
Posted: Mon Apr 23, 2012 8:52 am
by smartin
Hi!!
I'm devoloping an HPLC-UV method for the quantification of some tricyclic antidepressants.
I'm looking for a nice substance to be used as internal standard. I've read some papers that use Nordoxepin, but I'd like to find out some alternative.
Some suggestion???
Thank you!!!
Re: IS for Tricyclic Antidepressants
Posted: Tue Apr 24, 2012 12:26 pm
by smartin
Any suggestion????
Or somebody has a UPLC-UV method for clorimipramine, desclorimiprimamine cuantification?
Re: IS for Tricyclic Antidepressants
Posted: Tue Apr 24, 2012 2:32 pm
by AA
Have you considered using one of the tricyclic antidepressants that you are not anayzling for?
Re: IS for Tricyclic Antidepressants
Posted: Wed Apr 25, 2012 7:04 am
by smartin
Yes.... but I would like to use some another molecule....
Re: IS for Tricyclic Antidepressants
Posted: Wed Apr 25, 2012 7:32 am
by prepcolumn
There are several papers about quantification of tricyclic antidepressants in literaure..
What do you mean with nice substance? There are studies using bromazepam, ethyl-methyl amitriptyline etc.
Regards
Re: IS for Tricyclic Antidepressants
Posted: Thu Apr 26, 2012 2:11 pm
by smartin
I would like to use a cheap substance, with a good recovery, and that it's not simultaneously taken by the patient......
Re: IS for Tricyclic Antidepressants
Posted: Thu Apr 26, 2012 3:41 pm
by tom jupille
In the past, alkylphenones have been suggested as general-purpose internals standards (pick an appropriate chain length to get retention in the correct region of your chromatogram).