IS for Tricyclic Antidepressants

[smartin]

Hi!!

I'm devoloping an HPLC-UV method for the quantification of some tricyclic antidepressants.
I'm looking for a nice substance to be used as internal standard. I've read some papers that use Nordoxepin, but I'd like to find out some alternative.

Some suggestion???

Thank you!!!

[smartin]

Any suggestion????
Or somebody has a UPLC-UV method for clorimipramine, desclorimiprimamine cuantification?

[AA]

Have you considered using one of the tricyclic antidepressants that you are not anayzling for?

[smartin]

Yes.... but I would like to use some another molecule....

[prepcolumn]

There are several papers about quantification of tricyclic antidepressants in literaure..

What do you mean with nice substance? There are studies using bromazepam, ethyl-methyl amitriptyline etc.

Regards

[smartin]

I would like to use a cheap substance, with a good recovery, and that it's not simultaneously taken by the patient......

[tom jupille]

In the past, alkylphenones have been suggested as general-purpose internals standards (pick an appropriate chain length to get retention in the correct region of your chromatogram).