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Base sample solution and acid mobile phase

http://www.chromforum.org/viewtopic.php?t=19607

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Base sample solution and acid mobile phase

Posted: Tue Apr 10, 2012 8:54 am
by gys25016
Hi there,

I am new for HPLC. I have a question here. Currently I have an method which use ammonium hydroxide (0.1%) as sample preparation solvent but use 0.1%TFA water and 0.1%TFA in ACN as mobile phase. Column is Atlantis T3.

I wonder if there is any negative impact since one is base and another is acid.

Thank you in advance.

Re: Base sample solution and acid mobile phase

Posted: Tue Apr 10, 2012 2:36 pm
by tom jupille
As long as the injection volumes are small and your peaks are well-retained (i.e., away from t0) it shouldn't be an issue. If it is, it will probably manifest itself as baseline instability and/or peak shape problems.
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