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aceton 0.05%

http://www.chromforum.org/viewtopic.php?t=19590

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aceton 0.05%

Posted: Fri Apr 06, 2012 11:49 am
by kamel
Hi all,
some one can tel me why we use aceton 0.05% for HPLC gradient autovalidation? (I am using shimatzu HPLC).
and can I substitute water by MeOH (I have some dificulties to pass the test using aceton 0.05%/water).
thank you.

Re: aceton 0.05%

Posted: Fri Apr 06, 2012 3:16 pm
by Consumer Products Guy
Acetone is unretained, and it has a UV signal.

Re: aceton 0.05%

Posted: Mon Apr 09, 2012 11:45 am
by dblux_
Acetone is unretained, and it has a UV signal.

If "gradient autovalidation" is a test of pump and gradient proportioning valves then fact that acetone is unretained may be neglected, because test is executed with pressure restrictor instead of column.
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