column washing

[ambre]

Hi,

I'm using a small silica-NH2 column where I immobilized a proteoglycan. I inject a protein solubized in PBS but sometimes I have like a clog in my column.
Normally I wash it with 10% MeOH.
Do you know if it's the best way to wash it or do you use another solvent?? I'm affraid of damaging my column with my immobilization.

Thanks.

Amber

[tom jupille]

The general rule is to flush with something that is a good solvent for whatever gunk is in there. If the 10% MeOH is working, I would stick with that rather than risk killing your proteoglycan.