Chromatography Forum

Morning Everyone,
I've been running several assays on an Agilent Zorbax Eclipse Plus C18 column (2.1mm x 50mm x 1.8um) and i'm looking to try out similar columns for other manufacturers. Any suggestions? I was told Waters UPLC columns won't be compatible with my Agilent 1290 LC. Is that correct?
Thanks.

Chemboy831

Morning Everyone,
I've been running several assays on an Agilent Zorbax Eclipse Plus C18 column (2.1mm x 50mm x 1.8um) and i'm looking to try out similar columns for other manufacturers. Any suggestions? I was told Waters UPLC columns won't be compatible with my Agilent 1290 LC. Is that correct?
Thanks.
Chemboy831

UPLC columns are rated "just" up to 1000 bar where your 1290 can go up to 1200 bar. So just slow down your flow rate a bit.

As with any other column, use adjustable fittings so you can make a void free conection.
apart from this, they are columns like others.
(Of course you won't be able to use the Acquity eCord function... )

If you want to find similar (or totaly different) columns, have a look at the USP column equivalency database and use the PQRI model

Link: http://www.usp.org/app/USPNF/columns.html
>"compare columns" > scroll down to "PQRI database"
small "F" value = similar, big "F" = different

Thermo Fisher make similar columns (1.9 um). I think they are more similar to the one you use than the Acquity columns. The latter's stationary phase support is not pure silica but BEH, which changes "the game" a little bit.

Best Regards

There are 3 basic Acquity columns (< 2 um).

BEH - bridged ethyl hybrid
CSH - BEH with a charged surface (Charged Surface Hybrid)
HSS - High Strength Silica

There are a couple of C18 bondings within each particle.

Poke around the Waters web site for more details.

And yes, you can use them in a 1290 but watch the upper pressure limit (as was mentioned previously.

Hello,

I just saw your post for looking for an equivalent column for the Agilent Zorbax Eclipse Plus C18 column. Perhaps you should try Imtakt USA's Cadenza CD-C18 column. This column is a 3 micron column that offers 25-50% less back pressure, high column efficiency, better separation of hydrophobic compounds, higher steric selectivity, and a high plate count at a low flow rate. This column also comes in a HT/UP version....the HT version has a pressure limit of 500 bar, and the UP version has a pressure limit of 1000 bar. The HT/UP versions can be used on a UPLC system, you just have to by the universal end fittings from Waters.

More information about the Cadenza CD-C18 column and the HT/UP versions are below:

http://www.imtaktusa.com/site_media/fil ... CD_C18.pdf

http://www.imtaktusa.com/site_media/fil ... and_UP.pdf

For more information, please contact me at bverma@imtaktusa.com.

Hope this helps!

The HT/UP versions can be used on a UPLC system, you just have to by the universal end fittings from Waters.

User 'chemboy831' is operating an Agilent 1290 LC.

Imtakt's columns can be used on the Agilent system that you mentioned, you should not have to buy any end fittings.

UPLC columns are rated "just" up to 1000 bar where your 1290 can go up to 1200 bar. So just slow down your flow rate a bit.

Dear Hollow,
if I remember correctly, the recommendation (some years ago ) given by Waters for UPLC BEH columns below 1000 bar, only the HSS phases were specificated for 1000 bar, but I may be wrong on this.

Best regards
Klaus