Separation of 2-Chloro, 4-Fluorotoluene to its isomer

[praveenpaliwal]

Good Evening Experts,

I want to separate both of this isomers 2,4 CFT, (i.e 2-Chloro 4-Fluoro Toluene) and its isomer (4-Chloro,2 Fluoro Toluene) which condition is suitable for this????

Best regards

Praveen

[HPLCaddict]

I'd try to either use a PFP column, which usually shows unique selectivities for halogenated compounds, or some sort of C18 with a dense polymeric bonding (e.g. Nucleosil AB or Purospher STAR) which often are able to separate isomers because of their high steric selectivity.

[JMB]

You might also consider the porous graphitic carbon (e.g. Hypercarb) type column; these are advertised as very suitable for resolution of geometric isomers and diastereoisomers.

[praveenpaliwal]

I do not have the above described columns. Should I have use Atlantis T3 which have high silanophylic activity so that there is H- bond formation between analyte and active silanols may induced some separation.
One more thing should I have to use normal phase??

Praveen

[sepscientologist]

Your best bet is to try GC!

[praveenpaliwal]

I also agree for this On HPLC I got the sufficient separation but the run time is too long. I also want to switch the method on GC but which column is suitable for this ????

[Gerhard Kratz]

Hi Praveen,

Please check out the Website of RESTEK for applications on such compounds. good luck.

[praveenpaliwal]

Sir,

I have checked the RESTEK website but did not found any clue. Can u tell me another option regarding to this analysis.

Best regards

Praveen

[Gerhard Kratz]

Gas chromatographic determination of aromatic amines in water samples after solid-phase extraction and derivatization with iodine: I. Derivatization
Torsten C Schmidta, Corresponding author contact information,
Melanie Lessb,
Rainer Haasc,
Eberhard von Löwb,
Klaus Steinbacha,
Gottfried Storka
a Department of Chemistry, Philipps-University, Hans-Meerwein-Strasse, D-35032 Marburg, Germany
b Environmental Hygiene and Immunology, Medical Centre of Hygiene, Philipps-University, Pilgrimstein 2, D-35037 Marburg, Germany
c Bureau for Exploration of Hazardous Waste Sites and Pollution Research, Stadtwaldstrasse 45a, D-35037 Marburg, Germany
Maybe this will help.

[praveenpaliwal]

I just check all the information above given but it is just releated to Aromatic amines but how can I got the information about the separation of CFT isomers????

Praveen

[tristanewalters]

If you go to any of those sites that sell GC columns, I am sure if you talk to their technical support they will be able to point to in the right direction. Our lab typically uses restek or J&W (agilent) columns. There is probably a capillary column out there that would work for you. In the mean time I submitted a tech support request to see what they would say.

Edit: I would assume a DB-624 or DB-1 column would suit you just fine if you guys have them already.

[praveenpaliwal]

Thanks for all of your comments. I found the separation on GC column and also found the LOD around 0.1%.
As per most of your comments I tried the analysis on GC column and found very good separation.
Thanks all of you.

Best regards

Praveen