Chromatography Forum

Hi guys.

I have some questions about referenece wavelength, talking about DAD modules from Prominence and HP 1100.

First of all, some basics that I haven´t got really clear. When is it really necessary using it? Because I know when your solvents are meesing up your peak, or when there´s something in your sample matrix messing too.

And why 1100 let´s D2 lamp be reference until 400 nm, and Prominence 600 nm (because if you put a reference under 600, and turns on VIS lamp, gives you trouble)? There are 200 nm of difference. The range is wider. Don´t know if it is on the lamp technology or detector technology.

The generally accepted wavelength range of analytical utility for a deuterium lamp is 190-400nm(some say 360nm) since the UV energy drops to about 10% of the peak power at ~400nm.

Use of a reference wavelength rarely helps and is (in my understanding) primarily intended to correct for changes in refractive index of the mobile phase.