What role does Sodium Dedecyl Sulfate play in Mobile Phase
Posted: Thu May 12, 2005 4:43 pm
by Charissa
What role does Sodium Dedecyl Sulfate (SDS) play in Mobile Phase? I have a test method where SDS is added to acidified Methanol: Water (2:1). I would like to understand if SDS affects the retention time of HPLC Analysis using the above mentioned mobile phase? If it does, how?
Thanks in advance for any comments.
Posted: Thu May 12, 2005 5:33 pm
by tom jupille
SDS acts as an ion-pair reagent.
Do a search of the Forum using "ion pair" as the keywords and you will get a tremendous amount of information about the topic. Chris Pohl had a very informative post a few weeks ago (maybe a bit advanced for your question, though):
http://www.sepsci.com/chromforum/viewto ... t=ion+pair
Posted: Fri May 13, 2005 12:49 am
by Charissa
Hi Tom,
Thanks for the tips. I will certainly do a search using your suggestion.
Charissa