Chromatography Forum

Hi !

I'm new in this forum but I use to read the posts.
Today I would like to ask a question. I need to analyse an amino acid which the structure is near from glutamine in a plant extract.

I can elute it on the Zic pHILIC from SEQUANT with a retention time of 12 minutes, but in my plant extract I have another compound with a very near structure, and the two coelute.

So I would like to try in GC but I don't have a lot of experience in GC . I read that the amino acid can be derivatized with BSTFA in general doc on GC. I have also a DB 5 column.
Does anyone know conditions for the derivatisation ?
Which solvent compatible with BSTFA is used to dissolve the amino acid?

Thank you for help

Hello Mel, I have one procedure for amino acids derivatization. with HFBA
if you have your hidrolysis completed, you would have to:

Take 0.2ml of yuor sample ( or the equivalent to 1mg of protein)
Dry under N2 at 45 degrees
Ad 100ul of 3M AOcCl/isoButanol
incubete 20min at 120C
Dry under N2 at 45C
at HFBA 100ul
incubate againt and dry
disolve with Ethyl acetate.
and you should ready to inject.

Good luck!

Thank you very much !

I'll try !