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how to get rid HFBA contamination from lc/ms

http://www.chromforum.org/viewtopic.php?t=19381

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how to get rid HFBA contamination from lc/ms

Posted: Fri Mar 16, 2012 11:09 am
by danicrd
Hi at all, in our lab we have Thermo TSQ ultra :cry: Instrument is used for numerous method. Some of them use HFBA for ion pairing (amine in ESI+).
Now we have serious problem with negative ion mode: background of 213,259,427,169 m/z is 10e7 and sensitivity suffer.
I'm sure it's coming from hfba sticked somewhere in system. Anyone has a good cleaning method to overcome this problem?
I've already done some maint at my system like source cleaning, capillary change, etc.
Any help will be very appreciated.
Thank you

Re: how to get rid HFBA contamination from lc/ms

Posted: Fri Mar 16, 2012 11:54 pm
by MaryCarson
Do you have the option of replacing the whole LC?

Re: how to get rid HFBA contamination from lc/ms

Posted: Sat Mar 17, 2012 6:12 am
by danicrd
Only for test or definitely? In first case i can attach another pump, or another hardware without problem...we have some unused equipment..from jurassic park :cyclops:

Re: how to get rid HFBA contamination from lc/ms

Posted: Tue Mar 20, 2012 9:16 pm
by MaryCarson
I've heard HFBA hangs up in the degasser of the LC--so cleaning the MS isn't enough.
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