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ELISA PLATE READER LINEARITY

Posted: Thu Mar 15, 2012 12:09 pm
by carol
Dear all,

I don't know were to put this post but I think this is the best place. I am looking for standard solution to verified my plante reader, ELISA. The manual of the equipment states that OD test and lineary test need to be conducted, however, I don't know where to by these standard solution.

I will really appreciate any comment and suggestion,

Thanks in advance,
Carolina

Re: ELISA PLATE READER LINEARITY

Posted: Thu Mar 15, 2012 3:21 pm
by ScottHorn
What type of plate reader do you have? In my experience they are usually checked using a special plate, not a solution. It's basically a regular plate, but instead of wells full of solution, it has optical filters like those used to calibrate spectrophotometers. The manufacturer should be able to supply you with one.