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sample is doesn't fully dissolve and is a gel

Posted: Mon Mar 12, 2012 8:46 pm
by MestizoJoe
Hi,

I'm working with chitin. I want to get it fully dissolved in solution.

I made a 2 mg/mL mixture with 5% LiCl in DMA as the diluent. The material appears to be mostly transparent. I was hoping to remove large particles/aggregates/gels by filtering through 0.45 um membrane. This just clogged the membrane.

I was wondering if there is a way to remove these large particles/aggregates/gels and leave only the most soluble and best behaving species from chitin in solution.

I think the gelling and aggregation is due to greater molecular weight, but I am not sure of this.

Any help would be appreciated.

Re: sample is doesn't fully dissolve and is a gel

Posted: Tue Mar 13, 2012 3:14 am
by Alexandre
if you want to remove large particles try centrifugation