Chromatography Forum

Hello to everyone,
I have run various concentration of organic acids (0.1, 0.5, 1.0, 5.0 uM ) in GCMS after extracting and derivatizing to get a calibration curve.Igot the standard curve but the response ratio for some organic acids ( methylmalonic acid, glutaric acid, 3-methylglutaric acid, adipic acid and sebacic acid ) is 2,4,6..etc and the response ratio for some organic acids ( hexanoic acid, butyric acid, propionic acid,isovelaric acid and suberic acid) is 2.1 + e8, 4.1 + e8, 5.4 + e10...etc.Out of these two which calibration curve is correct.

Thanks
Chandra

This is a question that is probably better to ask your advisor or supervisor or something. You could also review the theory of calibration curves and that should answer your question: http://en.wikipedia.org/wiki/Calibration_curve

For things you want to quantify using the curve, they should fall on the linear region of the curve.