Tacrolimus-HPLC method

[Pranav]

Dear all
basicaly i am a research scholar of NDDS (Pharmaceutics)

Recently i am working on tacrolimus formulation development (by using lipid based drug delivery system). Right now i ma trying to set develop method of tacrolimus on HPLC with UV PDA detector (simple method, no need to separate isomer or metabolites). i have gone through several developed method and took the trial for the same, but unfortunately i am not getting desire result (initialy i got merzing of two peak after that i changed mobile phase and got one peak but it was wide) may be becuase of i cant control temperature of coloumn on my intstrument.

can anybody tell me how important to control temperature in case of tacrolimus and is there any other way to get the proper result by just selecting proper mobile phase????

Please give you few minutes and let me know your suggestion

Please do needful

Thanks
Pranav Patel

[AMIR HAIDER]

MOBILE PHASE:350ml acetonitrile 650ml water(4ml O-phosphoric acid in 600ml water)
UV detector:210nm
SAMPLE:prepared in acetonitrile
Column temparature:60 degree adjust
You also share your more experiences because its is our stable product

[DSP007]

System pH ?
Tarcolimus http://ru.wikipedia.org/wiki/%D0%A4%D0% ... eletal.png
contains an amide group
....... Amir say phase

[archie]

Hi all,
I am recently working on tacrolimus too...an ointment and cream.
What I got is the recovery is 10% lower...is anyone know the cause?
I'am using Zorbax C-8 column, Acetonitrile as solvent, and mixture of Methanol, acetonitrile and water as mobile phase.

@ Amir:
Is it really work? Because if I adding more than 20% water in mobile phase, I got wide and short peak, and the efficiency is just about 400..
What is the significance of using o-phosphoric acid in this case? because I dont see any peak tailing in the chromatogram.

Thanks a lot..

[harika]

hii

can any one help me in collecting the information regarding tacrolimus API hplc methods optimised till date?where exactly can i get such an information?

[bisnettrj2]

Ask Google, PubMed, or go to your local university's library and use their access to scientific journals.

[HW Mueller]

Chemical Abstracts of the American Chemical Society is still the standard for chemical information.

[harika]

thanku

[archie]

Dear all..

I want to ask you about my problem:
I have three kinds of tacrolimus ointment: 0.3%, 0.1% , and 0.03%.
When I am analyzing 0.3% ointment, I got 98.5%
When I am analyzing 0.1% ointment, I got 95%
When I am analyzing 0.03% ointment, I got 93.5%
Is anybody know the cause?

How can I be sure that the method is right?
Please give me some advices

Thanks a lot

[unmgvar]

did you check the linearity of the method?

[archie]

did you mean the linearity of the standard (like linearity in the method validation)?