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Rules for PLOT columns
Posted: Mon Mar 05, 2012 11:36 am
by Axar
Hello!
I need to do a method with plot column, but i never worked with this type of stationary phase. Does it have any special rules to work?
Thank you!!!
Re: Rules for PLOT columns
Posted: Mon Mar 05, 2012 3:20 pm
by chromatographer1
Sample size is less than packed columns. Watch the temperature limits.
Remember that the molecular size in PLOT columns also has a limit, usually around 6-8 carbons.
Involatiles on the column will plug the pores and cause tailing and loss of resolution.
wishing you all success,
Rod
Re: Rules for PLOT columns
Posted: Mon Mar 05, 2012 7:39 pm
by Bigbear
Ramp up the flow slowly so as not to displace the packing.
Re: Rules for PLOT columns
Posted: Wed Mar 07, 2012 5:25 am
by Axar
Thank you very much!!!