Ghost Peak after Column Washing with 0.1% TFA
Posted: Sun Feb 19, 2012 5:33 pm
Dear friends,
I was consistently getting 'ghost peak' and suspected contamination in my column {BEH Shield RP-18 (100x2.1) mm, 1.7 micron}.
I washed the column with A 90% to B 90% for a gradient time of ten minutes, in which A is 0.1% TFA in water and B is 0.1% TFA in acetonitrile. Unfortunately, instead of solving the problem, I got three more ghost peaks. They are now appearing even after washing with 10% aqueous ACN followed by 100% ACN.
Please Help!
I was consistently getting 'ghost peak' and suspected contamination in my column {BEH Shield RP-18 (100x2.1) mm, 1.7 micron}.
I washed the column with A 90% to B 90% for a gradient time of ten minutes, in which A is 0.1% TFA in water and B is 0.1% TFA in acetonitrile. Unfortunately, instead of solving the problem, I got three more ghost peaks. They are now appearing even after washing with 10% aqueous ACN followed by 100% ACN.
Please Help!