Reference standards for analysis of chemicals (Triton/TnBP)

[miro2009]

Hello chromatographers
I was wondering if anyone has experience with the folowing 2 chemicals as an analyte in a certain product:
Triton X-100
Tri n-butyl phosphate

We are developing chromatography methods (RP-HPLC and GC) for quantification of these chemicals but I was requested to use a certified reference of both as a reference for my method which I am unable to find in Merck, Sigma
Currently I am using the raw material itself that is used in our product which is of >99% purity, but it is not described it could be used as an chromatography reference, and it is not accepted by our QA.

Does anyone know if such chemicals are available as method references?

Thank you

[tlahren]

Tributyl phosphate
http://www.accustandard.com/ProductDeta ... -SP-1-100X

Triton X-100 solution. Not sure the concentration or certification. Maybe call them.
http://www.scbt.com/datasheet-29112-triton-x-100.html

Ty

[miro2009]

Tributyl phosphate
http://www.accustandard.com/ProductDeta ... -SP-1-100X

Triton X-100 solution. Not sure the concentration or certification. Maybe call them.
http://www.scbt.com/datasheet-29112-triton-x-100.html

Ty

Thanks a lot! I will order the tributyl phopshate, and luckily found a local supplier too.
As for the Triton, looking at the webpage it seems more like a reagant than a material to use as a reference.. I actually contacted accustandard hoping they also supply Triton as they are a certified company specialized for chemical references, otherwise, I'm still in need for a Triton X-100 proper standard!
Maybe anyone else has additional suggestions??

Thanks again.

[tlahren]

I find various different MWs listed for the Triton on different websites as it is technically a polymer. I'm sure because of this fact it will be hard to find a "certified" Standard for it.

At the contract lab I used to work for, we used to be allowed to analyze and report just about any compound as long as the data was flagged that we were not "Certified" to run it by the state. I'm not sure what type of business you are analyzing for (industry or environmental etc.) but this may be an option. You can order the bulk Triton X-100 and weight it out and dissolve it in Acetone or Methanol. If you do this from two different vendors you will be able to show that both standards are in agreement (second source standards are usually required for most environmental methods anyway). It still won't be "Certified" but you will have a lot of "in-house" QC to show that there is no vendor/lot bias.

I hope some of this helped you a bit.

Ty

[miro2009]

I find various different MWs listed for the Triton on different websites as it is technically a polymer. I'm sure because of this fact it will be hard to find a "certified" Standard for it.

At the contract lab I used to work for, we used to be allowed to analyze and report just about any compound as long as the data was flagged that we were not "Certified" to run it by the state. I'm not sure what type of business you are analyzing for (industry or environmental etc.) but this may be an option. You can order the bulk Triton X-100 and weight it out and dissolve it in Acetone or Methanol. If you do this from two different vendors you will be able to show that both standards are in agreement (second source standards are usually required for most environmental methods anyway). It still won't be "Certified" but you will have a lot of "in-house" QC to show that there is no vendor/lot bias.

I hope some of this helped you a bit.

Ty

Dear tlahren, our buiseness is a pharmaceutical company, where we analyze Triton in one of our products to ensure its not above a certain limit.. I want to go back to the way you dissolve it as I actually dissolve in water and not an organic solvent as you mention (I noticed also that the tributyl phosphate in accustandard is supplied dissolved in acetone), as our drug product is also of an aqueous base... is it preferred to dissolve triton in acetone/methanol for any specific reason?

[tlahren]

Just my default suggestion for GC analysis. If water works well for you then go with it. I have no other reasons really. I just generally stay away from water if I can for GC standards but this is usually method, column and detector specific. The TnBP in acetone should be no problem for either LC or GC analysis as you can dilute it with water or organic solvents for your calibration standards. My guess is that is why they sell it in acetone; it is compatible with many dilution solvents.

Ty