Chromatography Forum

Are there any experienced users of Beckman's 32 Karat software? We recently obtained a Beckman System Gold LC instrument. When we set up our method, which uses a 507 autosampler (set as external trigger in method as the manual instructs), and we get a message "waiting for trigger". No injection occurs. I am not sure if this is an issue with our method, or with the autosampler itself. In addition, the autosampler wheel doesn't home or move at all when we turn it off an on. Does it usually go to a home position? Thanks in advance for your help

Hi stchu,

I am support here at Beckman and can tell you that "Waiting for trigger" is just that. The software is telling you it needs an external or manual trigger signal (such as the one from the 507 autosampler) to launch and start the method. Make sure in your Instrument setup of the method portion that you go to your Injector tab ( would say Injector 507) and select the trigger status to "External". This allows the 507 to initate the start trigger itself rather than waiting for you to do it if its set to "manual". Also be sure its configured properly in the beginning of the software (right click on the instrument icon on the first screen of 32 Karat your normally double click on->select Configure->Instrument->Configure->Auto-Configure and make sure you get a detector, pump and autosampler icon on the right side of the window. Any other problem would be a problem with the autosampler itself and you can call 1-800-369-0333 to speak with me.

stev434

Hi Steve,

I am using a Beckman Gold System with a 508 Autosampler. I've got an error message saying that the the syringe did not make the right number of pulses when attempting to inject a sample and I wonder if you might possible advise about what is causing this error. Thanks in advance,

Maria