Esterification of acid in methanol mobile phase
Posted: Thu Jan 26, 2012 6:23 pm
Hello,
If I am preparing a 100% methanol mobile phase (assume it is dry) and I am adding an organic acid as a modifier, does anyone know how fast the esterification reaction will be (formation of methyl formate and concomitant reduction in the amount of formic acid)? I know it depends on acid concentration, etc. but if anyone has any guidelines or a reference I would appreciate it.
Chris
If I am preparing a 100% methanol mobile phase (assume it is dry) and I am adding an organic acid as a modifier, does anyone know how fast the esterification reaction will be (formation of methyl formate and concomitant reduction in the amount of formic acid)? I know it depends on acid concentration, etc. but if anyone has any guidelines or a reference I would appreciate it.
Chris